三岔子林区黄檗野生种群资源状况的调查与分析

以吉林省三岔子林业局不同林龄的林分为对象,调查与分析黄檗野生种群的资源状况,结果表明:单位面积活立木平均株数在幼龄林林分中占12.3%,在中龄林中占3.8%,在成过熟林中占2.6%,3个龄组林分的平均株数比例为6.2%;单位面积活立木平均蓄积在幼龄林林分中占7.3%,在中龄林中占2.4%,在成过熟林中占1.3%,3个龄组林分的平均蓄积比例为3.7%。     

和龙市林业局龙牙楤木的开发利用

龙牙楤木是享誉长白山地区最著名的木本山野菜之一，除了食用价值，还兼有补气安神、强精滋肾的药用价值。由于当地广大群众一直实行掠夺性的采摘方法，致使野生龙牙楤木资源受到严重破坏，其蕴藏量和可采量逐年减少，供需矛盾日渐突出；为保护当地濒危经济植物———龙牙楤木，采取必要的保障措施，综合开发利用，才能使龙牙楤木产业真正成为和龙市多种经营的重要来源之一。     

基于26个微卫星标记的三江水系草鱼遗传多样性分析

选取26个微卫星标记对来自长江(监利、邗江)、黑龙江、珠江3个水系的4个野生草鱼(Ctenopharyngodon idellus)群体的遗传多样性进行了检测。结果表明,26个微卫星位点均为高度多态位点,草鱼4个地理群体的多态信息含量(PIC)平均值为0.581 3~0.638 6;共检测出141个等位基因,其中有102个为共有等位基因;每个位点有等位基因2~11个,平均等位基因5.46,平均有效等位基因3.455 6。4个地理群体野生草鱼的平均杂合度在0.711 4~0.804 5之间。群体间遗传固定指数(FST)及AMOVA分析表明,群体间遗传分化并不显著(FST=0.031 73)。基于DA遗传距离构建的UPGMA聚类树表明,监利群体与邗江群体这两个长江水系野生地理群体聚为一支,然后与珠江群体聚为一支,黑龙江群体单独聚为一支。长江群体是原始群体。综上所述,三江水系野生草鱼具有较高的遗传多样性,4个群体之间遗传分化并不明显。     

汉中市野生兰科植物资源现状及保护开发策略

汉中市共分布野生兰科植物34属57种,分别占到陕西省的87.8%和68.3%,具有较高观赏价值的有兰属中的春兰和惠兰、独蒜兰属的独蒜兰,具有较高药用价值的有传统贵重中药天麻、白芨等。汉中市野生兰科植物总体资源量大、分布广,有着悠久的兰科植物栽培和开发利用历史,但近年来由于盗挖滥采现象较为严重,建议加强保护,建立野生兰科植物基因库,并加强人工栽培进行开发性的保护。     

牡丹杂交育种试验研究

在建成中国野生牡丹种质资源圃的基础上,利用野生牡丹与各栽培品种进行杂交育种,试图将野生种的优良基因引入栽培品种中,以培育出优良园艺新品种。主要杂交组合有：（1）野生牡丹之间（正反交）;（2）野生牡丹与各类栽培品种群间（正反交）;（3）西北牡丹品种群与中原牡丹品种群间。目前已得到了14批杂种及实生苗,从中选育出了50多个优良新品种。     

基于GIS的野生哺乳动物空间平衡抽样方法研究——以南京东郊为例

城市近郊大型绿地的野生动物生态监测方法研究是一个普遍而尚未引起足够重视的课题。空间平衡抽样结合了简单随机抽样和系统抽样2种抽样方法的优点,对点状、线状、面状不同类型的调查对象具有较强的适应性。以南京东郊的大型哺乳动物河麂Hydropotes inermis为研究对象,以47个物种痕迹点和江苏基础地理信息数据库的8个环境变量为主要信息源,采用最大熵法(Maxent)生态位模型,建立潜在生境预测模型。在此基础上,分别采用反向随机四分递推栅格(RRQRR)、通用随机方格分层算法(GRTS)2种算法,进行空间平衡抽样,从生境适生概率、监测成本、调查难度3个方面建立评价指标来比较空间平衡抽样与系统抽样、随机抽样性能的优劣。结果表明:南京东郊河麂的潜在生境呈高度破碎化格局,距离公路距离(41.9%)、距离铁路距离(17.8%)、距离居民点距离(14.7%)3个与快速交通有关的因子的贡献率占了74.4%;当样本大小为200时,空间平衡抽样的抽样对象样本点平均适生概率平均为0.029 850 5,分别比简单随机抽样、系统抽样高了77.34%、68.30%,显示了较高的抽样精度;平均海拔、平均坡度分别为86.927 8 m、1...     

Utilization of genetic resources for the introduction and adaptation of exotic vegetable crops: The case of pepino (Solanum muricatum)

Summary Pepino (Solanum muricatum), a vegetatively propagated plant from the Andean region used for its edible fruits, has been identified as a potential crop for greenhouse cultivation in Mediterranean regions. However, attempts for introducing it have been unsuccessful, either because of the low yield, poor fruit quality, or both. Screening of germplasm under Mediterranean conditions showed that sources of variation for high yield existed in the cultivated genepool and that wild species S. caripense and S. tabanoense could contribute to a considerable improvement of soluble solids content (SSC) of pepino. Progenies obtained after crossing genetically distant (AFLP-based) parental clones were heterotic for yield, and allowed the selection of clones with an improved combination of yield and SSC. As a result of this intraspecific programme, two new improved cultivars (‘Turia’ and ‘Valencia’), which outperform the rest of cultivars available, have been selected for cultivation in Mediterranean conditions. The interspecific programme involved the selection of clones with high yield and SSC in backcross generations. The results show that introgression of genes from the wild species can contribute to improve the SSC of pepino. The backcross programme is in an advanced stage and new cultivars derived from the interspecific crosses are expected to be released in a near future. New prospects for the future in pepino breeding include the genetic transformation, the establishment of a genetic map, and the use of the genomic information from related Solanaceae important crops. All the information obtained is illustrative for the breeding for adaptation of vegetatively propagated crops.     

Genetic diversity of wheat wild relatives in the Near East detected by AFLP

In order to reveal the molecular genetic diversity of wheat wild relatives, an AFLP analysis was conducted with 16 accessions of five Triticum andAegilops species originating from the Near East. Variation within population was studied with at least seven individuals per accession. Four primer combinations were used for selective amplification. Based on the scored bands, we estimated percentage of polymorphic bands, 1 – proportion of shared bands (1-psb) and nucleotide diversity (π). Of the five species used in this study, Ae. speltoides had the highest level of `within population' variation. This species had also the highest value of the variation among populations. As for Triticum species, the level of variation within population was low in diploid species (T. urartu and T. boeoticum),whereas two tetraploid species (T. dicoccoides and T. araraticum) had relatively high levels of variation within population. While the two diploid Triticum indicated a clear interspecific divergence, the two tetraploid wild wheats were not clearly divergent in this study. The variance portioning analysis indicated that the variation detected for diploid Triticum species was mainly composed of `between species' variation, on the other hand that for tetraploid Triticum was mostly composed of `within population' variation. In conclusion, AFLP analysis reveals molecular variation in all accessions used in this study, suggesting a potential genetic diversity of the wheat wild relatives in natural populations. These results have implications for the design of strategies to maintain genetic diversity within genebank collections. This revised version was published online in August 2006 with corrections to the Cover Date.     

Evaluation of genetic variation among wild rice populations in Cambodia

A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources.     

玉米间作菊花脑高效栽培方法对玉米杂草的影响

研究了玉米间作菊花脑的高效栽培方法对玉米杂草的影响,以探究此间作方法对杂草的防效及间作经济效益,为在生产实践中进一步推广应用提供理论依据。结果表明,玉米菊花脑间作处理(T)较玉米单作处理(CK),玉米产量增加,每667 m2产值增加2496元,增收效果明显;间作处理的玉米维生素C、粗淀粉、可溶性糖、可溶性蛋白含量分别提高了41.7%、3.6%、6.2%、14.2%;在玉米各时期CK处理杂草发生种类和相对密度均高于T处理;玉米成熟期T处理防草效果最好,株数防效和鲜质量防效分别达87.64%和90.28%,有效减少杂草生长。综上所述,玉米间作菊花脑的高效栽培方法有效减少了除草剂的用量,菊花脑经济效益更高,能在保证玉米产量的同时,显著提高农民收入。